Protein_Domain
rigid
Part:BBa_K1362439:Design
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-08)
rigid linker for λ-Lysozyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This Linker has the amino acid sequence GGAEAAAKEAAAKAAP. AEAAAKEAAAKA [1] forms a rigid alpha helix. GG is a flexible spacer sequence. The P was added to make sure that the helix is disrupted.
Source
This sequences was obtained by DNA synthesis.
References
[1] Arai, R., Ueda, H., Kitayama, a, Kamiya, N. & Nagamune, T. Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng. 14, 529–32 (2001).